Andrews Lab

Our Lab

13th floor garden stairs

Our lab is located in The Donnelly Centre for Cellular and Biomolecular Research at the University of Toronto. We have access to several state-of-the-art facilities including robotic facilities, high content screening microscopes and sequencing facilities.

Selected publications

PIFiA: Self-supervised Approach for Protein Functional Annotation from Single-Cell Imaging Data.: Razdaibiedina, A, Brechalov, A, Friesen, H, Mattiazzi Usaj, M, Masinas, MPD, Garadi Suresh, H, Wang, K, Boone, C, Ba, J, Andrews, B, 2024, Mol Syst Biol. in press, bioRxiv 2023.02.24.529975
Proteome-scale movements and compartment-connectivity during the eukaryotic cell cycle.: Litsios, A, Grys, B, Krause, OZ, Friesen, H, Ross, C, Masinas, MPD, Forster, DT, Couvillion, MT, Timmerman, S, Billman, M, Myers, C, Johnsson, N, Churchman, S, Boone, C, Andrews, BJ, 2024 Cell, 187, 1490–1507 https://doi.org/10.1016/j.cell.2024.02.014
Exploring whole-genome duplicate gene retention with complex genetic interaction analysis.: Kuzmin E, VanderSluis B, Nguyen Ba AN, Wang W, Koch EN, Usaj M, Khmelinskii A, Usaj MM, van Leeuwen J, Kraus O, Tresenrider A, Pryszlak M, Hu MC, Varriano B, Costanzo M, Knop M, Moses A, Myers CL, Andrews BJ, Boone C. 2020, Science, 368(6498):eaaz5667.
Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.: Mattiazzi Usaj M, Sahin N, Friesen H, Pons C, Usaj M, Masinas MPD, Shuteriqi E, Shkurin A, Aloy P, Morris Q, Boone C, Andrews BJ. 2020, Mol Syst Biol., 16(2):e9243.
Global Genetic Networks and the Genotype-to-Phenotype Relationship.: Costanzo M, Kuzmin E, van Leeuwen J, Mair B, Moffat J, Boone C, Andrews B. 2019, Cell, 21;177(1):85-100.
Exploring genetic suppression interactions on a global scale.: van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, Ušaj MM, Pechlaner M, Takar M, Ušaj M, VanderSluis B, Andrusiak K, Bansal P, Baryshnikova A, Boone CE, Cao J, Cote A, Gebbia M, Horecka G, Horecka I, Kuzmin E, Legro N, Liang W, van Lieshout N, McNee M, San Luis BJ, Shaeri F, Shuteriqi E, Sun S, Yang L, Youn JY, Yuen M, Costanzo M, Gingras AC, Aloy P, Oostenbrink C, Murray A, Graham TR, Myers CL, Andrews BJ, Roth FP, Boone C. 2016, Science, 354(6312).
Exploring Quantitative Yeast Phenomics with Single-Cell Analysis of DNA Damage Foci.: Styles EB, Founk KJ, Zamparo LA, Sing TL, Altintas D, Ribeyre C, Ribaud V, Rougemont J, Mayhew D, Costanzo M, Usaj M, Verster AJ, Koch EN, Novarina D, Graf M, Luke B, Muzi-Falconi M, Myers CL, Mitra RD, Shore D, Brown GW, Zhang Z, Boone C, Andrews BJ. 2016, Cell Syst., 3(3):264-277.e10.
A global genetic interaction network maps a wiring diagram of cellular function.: Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C. 2016, Science, 353(6306).

Research

Genomics and Genetics: Since we have mapped the entire set of digenic genetic interactions in budding yeast, our lab now uses synthetic genetic array (SGA) and CRISPR technology to explore a range of more complex genetics questions. We look at trigenic interactions, genetic interactions in the presence of chemical stress, complex haploinsufficiency, and genetic suppression in yeast. We create a variety of resources, databases and collections for the community with application to the SGA platform, including sets of CRISPR-based tools (1) for generating mutant collections in other yeast backgrounds and (2) for generating and phenotyping collections of yeast mutants using array-synthesized oligos. We have built several collections – most recently the YETI collection, with every gene under the control of a titratable estradiol promoter. In association with the lab of Jason Moffat at the CCBR, we are using a human cell line, HAP1, and the lentiviral TKOv3 library to explore genetic interactions, chemical-genetic interactions and associations with various disease genes.
Biological Discovery Using High Throughput Imaging: We combine SGA with high content screening (HCS), using automated image acquisition and analysis, in two types of studies. In one, we screen collections of mutants to identify those that show defective morphology in different GFP-tagged compartments and organelles. In the second type of study, we image collections of GFP-tagged proteins to assess changes in protein localization and abundance. We have done this across the cell cycle, as cells die, and in a variety of mutant backgrounds and environmental conditions.
Computational Analyses: In collaboration with computational researchers at the Donnelly and beyond, we are developing algorithms to integrate diverse biological networks, to systematically identify aberrant cells in an image, and to identify subcompartmental localizations of proteins in an image.