Protocols, Data and Resources


  1. Synthetic Genetic Array (SGA) protocol
  2. Reporter-SGA
  3. Genome-wide Synthetic Dosage Lethal (SDL) screening (Coming soon)
  4. High Content Screening (SGA-HCS) (Coming soon)


  1. Yeast Kinase Interaction Database (Yeast KID)
  2. Data repository of high-content cell imaging projects (TheCellVision)
  3. Collection of Yeast Cell and Localization Patterns (CYCLOPS)
  4. Data Repository of Yeast Genetic Interactions (TheCellMap)
  5. Database of protein and genetic interactions (BioGRID)

Over-expression Libraries and Collections

Our lab has a series of overexpression collections in both yeast and E-coli. We are happy to provide you with specific strains or plasmids upon request. The GST and mORF libraries are available in full through Open BioSystems. Not all genes are available in all collections. To search for your favourite gene’s availability, please click on the icons below to download the appropriate EXCEL files. When requesting a strain, please provide the name of the collection, the ORF and gene name, whether you would like the bacterial or yeast strain, as well as the coordinates from the requested library. To receive strains from the MoBY collection, please contact Dr. Charlie Boone’s lab.

Collection Copy# Promoter Sequence Verified? Tag # of ORFs Yeast Marker Yeast/E-coli
Sopko et al, 2006
Zhu et al, 2000
High 2µ GAL1/10 inducible Some N-terminal GST 5280 URA3,leu2d Yeast & E-coli
Gelperin et al, 2005
High 2µ GAL1/10 inducible Yes (both ends of ORFs) C-terminal His6-HA-ProteinA 5854 URA3


Hu et al, 2007
Low CEN GAL1/10 inducible Yes (fully verified) None 5200 URA3

Gateway Barcoded

Yeast & E-coli
MoBY (Ver 1.0)
Ho et al, 2009
Low CEN Native Yes (3' end of ORFs and both barcodes) None 5037 URA3

MAGIC Barcoded

Yeast(254) & E-coli
MoBY (Ver 2.0)
Magtanong et al, 2011
High 2µ Native Yes (3' end of ORFs and both barcodes) None 4527 LEU2

MAGIC Barcoded